Concentrations of PGE2 were measured in medium of the amnion- and decidua-facing chambers of Transwell cultures of human gestational membranes after 1, 4, 8 and 24 h of exposure to 100 ng/ml LPS (added to both the amniotic and decidual chambers of the cultures). Concurrent controls were exposed to culture medium without LPS. A) PGE2 concentrations in amniotic chamber medium. B) PGE2 concentrations in decidual chamber medium. Values are expressed as mean ± SEM from triplicate cultures for each subject (n=6, 8, 11 and 4 subjects at 1, 4, 8 and 24 h, respectively). *Significantly different from time-matched controls (p< 0.05). #Significantly different from time-points indicated within treatment group. LPS, lipopolysaccharide; PGE2 prostaglandin E2.