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. 2003 Dec;23(24):9014–9024. doi: 10.1128/MCB.23.24.9014-9024.2003

FIG. 9.

FIG. 9.

Dox-induced expression of NFIen inhibited SP-C expression but not TTF-1 expression in transgene-positive regions of lung parenchyma. Adult double-transgenic mice were fed normal (A to D) or Dox-treated (E to H) food for 8 days. Anti-HA IHC was used to detect HA-tagged NFIen transgene expression. NFIen was not detected in double-transgenic mice that were fed normal mouse chow (A) but was readily detected in a subset of TII cells in double-transgenic mice that were fed Dox for 8 days (E). TTF-1 was detected in the nuclei of TII cells in both transgene-positive and transgene-negative areas (B and F). SP-C mRNA was detected by ISH on serial sections of the same regions used for IHC, viewed by dark-field (C and G) and phase-contrast (D and H) optics. Arrows indicate representative IHC-positive TII cells. Bar = 50 μm.