Figure 5.

Constitutive high expression of heat shock proteins and chaperones is not sufficient to adapt to the loss of SRP⁵⁴. Accumulation of untranslocated Kar2 upon induction of SRP54^dn was measured in a wild-type strain (SMY211; □; n = 4) and in a strain containing a constitutively active allele of HSF1 (SMY226 [HSF1^C; hsf::LEU2, with pHF35]; ▪; n = 2). Cells were grown in galactose-containing medium for 4 h, labeled, and processed for immunoprecipitation with anti-Kar2 as described in Figure 1. The precursor form of Kar2 is plotted as percentage of total Kar2 immunoprecipitated.