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Primary antibodies used in this study. Antibodies to each protein component of the pre-incision complex are listed along with the epitope of recognition (where available), and the working concentration used. Suppliers are: N.B., Novus Biologicals; N.M., NeoMarkers; S.C., Santa Cruz Biotechnology. In studies where whole cell extracts (WCEs) were used, the monoclonal XPA antibody (clone 12F5) provided an increased signal:noise ratio, and was thus used for all WCE analyses.
