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. 2011 Jun 15;14(12):2465–2477. doi: 10.1089/ars.2010.3369

FIG. 9.

FIG. 9.

Detection of other NER pre-incision complex proteins and platinum preference for binding to dsDNA. (A) Detection of ERCC4/XPF, TFIIH, XPG, and ERCC1 from WCEs. Extracts were prepared from HeLa cells and the indicated amount of total protein was incubated with large fragments of platinated or nonplatinated dsDNA. (∙) ERCC4/XPF, (□) TFIIH, (▴) XPG, and (◊) ERCC1. (B) Detection of platinum preference for NER proteins: RPA, XPA, ERCC1, XPG, TFIIH, ERCC4/XPF, and XPC. Binding to platinated dsDNA (black) and non-platinated dsDNA (gray). *p = 0.01, **p > 0.05.