FIG. 16.

(A) The C-terminal sequence alignment of the AIF-like proteins and (B) the x-ray structure of human AIF. (A) A DNA-binding helix-turn-helix motif predicted in D. discoideum is boxed. Residues corresponding to the ⁵¹²Lys-Arg-Arg⁵¹⁴ cluster in D. discoideum are also indicated. (B) The 595–605 peptide corresponding to the DNA-binding motif in D. discoideum is displayed in black. Instead of the basic cluster predicted to comprise the turn between helices in D. discoideum, human AIF has ⁵⁹⁴Asp-Gly-Glu⁵⁹⁶, which makes the surface more acidic and less suitable for DNA binding.