Table 1.
Strains and Plasmids Used in This Study
| Strain name | Genotype/insert | Notes |
|---|---|---|
| ER1821 (wild type) | F−glnV44 e14−(McrA−) rfbD1? relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10 | New England Biolabs |
| RGP209 | ER1821 dsbC− | Lab stock |
| RGP433 | ER1821 dsbC− dsbB− | Lab stock |
| RGP663 | ER1821+ pPDI detector | This study |
| RGP664 | ER1821 dsbA−+ pPDI detector | This study |
| RGP665 | RGP209+ pPDI detector (the starting strain) | This study |
| RGP666 | ER1821 dsbD−+ pPDI detector | This study |
| RGP667 | ER1821 dsbA−dsbC−+ pPDI detector | This study |
| RGP668 | ER1821 trxA−+ pPDI detector | This study |
| RGP669 | ER1821 trxB−+ pPDI detector | This study |
| RGP181 | ER1821+pBR322 | This study |
| RGP188 | ER1821 dsbA−+pBR322 | This study |
| RGP195 | ER1821 dsbC−+pBR322 | This study |
| RGP286 | ER1821 dsbD−+pBR322 | This study |
| RGP202 | ER1821 dsbA−dsbC−+pBR322 | This study |
| RGP655 | ER1821+ pBla 81-108 | This study |
| RGP685 | ER1821+ pBla 52-81 | This study |
| RGP686 | ER1821+ pBla 98-108 | This study |
| RGP719 | ER1821+ pBla 52-108 | This study |
| RGP720 | ER1821+ pBla 81-98 | This study |
| RGP829→834 | UVC49→54, dsbC− suppressor strains | This study |
| RGP843→848 | UVC56→61, dsbC− suppressor strains | This study |
| RGP852 | UVC62, dsbC− suppressor strains | This study |
| RGP877→880 | UVC63→66, dsbC− suppressor strains | This study |
| RGP849 | UVC49 cured the pBR322 plasmid | This study |
| RGP851 | UVC52 cured the pBR322 plasmid | This study |
| RGP1079 | ER1821 dsbB1-148 dsbC−(dsbB was truncated as in UVC56) | This study |
| RGP1126 | ER1821 dsbB1-148dsbC− cpxRM23I,E24K(kdgT::kan) | This study |
| RGP1146 | RGP1126 kan cassette removed | This study |
| RGP929 | RGP433+pPDI detector | This study |
| RGP982 | RGP1079+ pPDI detector | This study |
| RGP916 | UVC56 dsbA− | This study |
| RGP917 | UVC56 dsbB− | This study |
| RGP918 | UVC56 dsbD− | This study |
| RGP919 | UVC56 dsbG− | This study |
| RGP920 | UVC56 umuD::kan (dsbB+) | This study |
| RGP1073 | UVC56 kdgT::kan (cpxR+) | This study |
| RGP1071 | UVC56 umuD−(dsbB+) kdgT::kan (cpxR+) | This study |
| RGP1181 | ER1821+ pRDB8-a | This study |
| RGP1182 | RGP209+ pRDB8-a | This study |
| RGP1183 | UVC49+ pRDB8-a | This study |
| RGP1184 | UVC52+ pRDB8-a | This study |
| RGP1185 | RGP1126+ pRDB8-a | This study |
| RGP1244 | RGP209+ pRDB8-a+pBADDsbA | This study |
| RGP1246 | RGP1079+ pBADDsbA | This study |
| RGP860 | ER1821+pMPB | This study |
| RGP862 | RGP209+pMPB | This study |
| RGP856 | UVC49+pMPB | This study |
| RGP858 | UVC52+pMPB | This study |
| RGP1130 | RGP1126+pMPB | This study |
| RGP1256 | UVC56 degP− | This study |
| RGP1269 | RGP665 yigZ::kan49 (dsbA loci from UVC49) | This study |
| Plasmid name | ||
|---|---|---|
| pBR322 | With wild-type β-lactamase (Bla) in the vector | Lab stock |
| pPDI detector | pBR322, Bla with mutations of S81C (TCA to TGT) and T108C (ACT to TGT) | This study |
| pBla 81-108 | pBR322 with Bla C52S C98S S81C T108C | This study |
| pBla 52-81 | pBR322 with Bla C98S S81C | This study |
| pBla 98-108 | pBR322 with Bla C52S T108C | This study |
| pBla 81-98 | pBR322 with Bla C52S S81C | This study |
| pRDB8-a | Urokinase constitutively active in the periplasm | Lab stock |
| pMPB | MBP and BPTI fusion protein in the periplasm | This study |
| pETDsbA | Codon optimized DsbA sequence | Lab stock |
| pBADDsbA | DsbA expression induced by arabinose | This study |
BPTI, bovine pancreatic trypsin inhibitor; MBP, maltose binding protein.