Table 2.

Ampicillin Sensitivity of the yihG::kan P1 Transduced Suppressor Strains

Amp (g/L)	WT	dsbC−	49	50	51	52	53	54	56	57	58	59	60	61	62	63	64	65	66
0.2	6	6	6	6	6	6	6	6	6	6	6	3	6	4	6	6	6	6	6
1.5	6	0	0	0	0	0	0	0	6	0	0	0	0	0	0	0	0	0	0

Growth on plates containing 0.2 g/L ampicillin was used to indicate the presence of the PDI detector plasmid. Growth of the same strains on plates containing 1.5 g/L ampicillin was used to indicate isomerase activity. The values in the 0.2 g/L ampicillin row indicate the tested colonies and those in the 1.5 g/L row indicate the survivors. Only the wild-type and UVC56 strains remained resistant to 1.5 g/L ampicillin after the dsbA region was substituted with wild-type DNA by yihG::kan cotransduction using P1 phage (100% linkage).

Amp, ampicillin.