FIG. 5. .

Relative levels of enhancement of interleukin-15 (IL-15) translation in UV-irradiated RM-1α and B16α vaccine cells. Monolayers of RM-1α cells and B16α cells were grown in medium containing 3,000 units/mL of interferon-α (IFN-α) for 15 days. Control monolayers of parental cells were given medium alone without IFN treatment. At 24 h after providing fresh medium with or without IFN-α, cell monolayers were subjected to lethal UV irradiation, left alone without UV treatment, or harvested for sonication. Another 24 h later, the remaining cell monolayers were harvested for sonication. The sonicates were assayed for the presence of IL-15 by ELISA. Standard curves of IL-15 were run concomitantly with the samples. The data are plotted as percent of untreated control cells. Data for non-irradiated and UV-irradiated B16 and B16α melanoma cells are also plotted. The data are expressed as percent of no UV 0 h control (RM-1 cells or B16 cells) divided by 100. ANOVA analysis for intracellular IL-15 in the RM-1 and RM-1α prostate cancer cell groups: no UV 0 h RM-1 parental cells versus no UV 24 h RM-1 parental cells versus UV 24 h RM-1 parental cells: P = NS; no UV 0 h RM-1 parental cells versus no UV 0 h RM-1α cells: P = NS; no UV 24 h RM-1 parental cells versus no UV 24 h RM-1α cells: P < 0.0001; UV 24 h RM-1 parental cells versus UV 24 h RM-1α cells: P < 0.0001; no UV 0 h RM-1α cells versus no UV 24 h RM-1α cells: P < 0.0001; no UV 24 h RM-1α cells versus UV 24 h RM-1α cells: P < 0.0001. ANOVA analysis for intracellular IL-15 in the B16 and B16α melanoma cell groups: no UV 0 h B16 parental cells versus no UV 24 h B16 parental cells versus UV 24 h B16 parental cells: P = NS; no UV 0 h B16 parental cells versus no UV 0 h B16α cells: P < 0.0001; no UV 24 h B16 parental cells versus no UV 24 h B16α cells: P < 0.0001; UV 24 h B16 parental cells versus UV 24 h B16α cells: P < 0.0001; no UV 0 h B16α cells versus no UV 24 h B16α cells: P = 0.021; no UV 24 h B16α cells versus UV 24 h B16α cells: P = NS.