FIG. 5. .

Quantification by spectrofluorimetric analysis of Alexa ovalbumin (Ova) in the tissue extracts at various times following subconjunctival ReGel injections in rat eyes. Two independent, identical experiments were conducted and both sets consisted of 20 μL injections of ReGel at 2 mg ovalbumin/mL ReGel (40 μg ovalbumin total). Three eyes from 2 rats were used in both experimental sets, providing a total of 6 eyes for tissue analysis at each time point. Tissues were dissected from the eyes at several time points and the ovalbumin in the tissue was extracted and measured by spectrofluorimetry. The amount of ovalbumin (ng) extracted from tissue homogenates of retina (A), choroid/RPE (B), and sclera (C) per eye as a function of time is shown. Each data point represents the mean ± SD from 3 eyes (n = 3) per experimental set. Two duplicate sets of experiments were performed and are reported separately giving a total of n = 6 at each time point.