Table 1. .
Validation Parameters for the HPLC Method of Analyzing Steroids
| Drug |
Linearity |
LOQ ng/mL | LOD ng/mL | Range μg/mL | Repeatability (%) | Accuracy (%) | ||
|---|---|---|---|---|---|---|---|---|
| Slope | Constant | R2 | ||||||
| DEX | 166197 | 193671 | 0.9994 | 700 | 200 | 0.78–6.25 | 1.68–6.60 | 95.50–98.90 |
| HA | 201599 | −22003 | 0.9991 | 750 | 250 | 0.78–6.25 | 1.42–4.21 | 96.80–102.31 |
| PA | 157138 | 355428 | 0.9951 | 750 | 250 | 0.78–6.25 | 2.11–3.80 | 98.31–103.65 |
Abbreviations: DEX, dexamethasone; HA, hydrocortisone acetate; HPLC, high-performance liquid chromatography; PA, prednisolone acetate.