Table 1. .

Description of Datasets Used in the Experiments

MTP Dataset Name	Data Source
Synthetic1	Synthetic data created in Microsoft Excel with a background SD of 5%, containing hits less than or equal to 5 × SD (Fig. 3A)
Synthetic2	Synthetic data created in Microsoft Excel to represent edge effects in the 3 most peripheral wells (Fig. 4A)
Synthetic3	Synthetic data created in Microsoft Excel to represent edge effects in the most peripheral wells (within 3 from the edge), with hits created by a random multiplier or divisor in the range 1–10 (Fig. 5A)
Experimental1	eGFP Readout of MIN6 cells incubated for 2 days and imaged on an INCell1000 imager (Fig. 4D) with no hits implemented
Experimental2	eGFP Readout of MIN6 cells incubated for 2 days and imaged on an INCell1000 imager. Up hits were simulated using phalloidin-FITC for illustration in Figure 6 and analysis presented in Figure 7
Experimental3	Data are eGFP or DsRED (2 colors, same MTP) readout of MIN6 cells on an Envision plate-reader after incubation for 5 days
Experimental4	eGFP or DsRED readout of MIN6 cells on an Envision plate-reader (Fig. 5D and 5G, respectively) after incubation for 5 days. Down hits were simulated by addition of methanol as described in Methods.

 The datasets listed are created and used for gauging the performance of the correction methods as described in the Methods.