TABLE 1.
Effect of single omission of initiation factors of the helicase complex on the yield of 48S complexes assembled on mRNAs bG and Td3
| Factor omitted | Relative toeprinting efficiency (%)
|
|
|---|---|---|
| bG | Td3 | |
| None | 100 | 100 |
| eIF4Aa | 73 | 25 |
| eIF4Bb | 80 | 20 |
| eIF4F | 13 | <10c |
a
In this case, the system contained ∼110 nM eIF4A as a eIF4F subunit compared to its usual concentration of 550 nM of free eIF4A and 110 nM of eIF4A from eIF4F.
b
It was not possible to exclude eIF4B completely due to its slight contamination of the eIF4F preparation (final concentration ∼20 nM compared to regular 300 nM).
c
It's difficult to define an exact value because the relative error is quite large when the signal is only slightly above background.