Figure 2.

Effects of α-tocopherol, α-tocotrienol, and tocotrienol rich fraction (TRF) on collagen-induced platelet aggregation: α-Tocopherol (250 μg), α-tocotrienol (250 μg) or TRF (250 μg) were dissolved in polyethylene glycol (1 mL) solvent and stored at -20°C. Plasma (40 mL) was warmed to 50°C (20 min) prior to injection. α-Tocopherol, α-tocotrienol or TRF solutions in 1 mL of polyethylene glycol were added to warmed plasma, vortexed vigorously, and then administered over 30 seconds through a femoral arterial line to anesthetized dogs. Collagen (12.5 μmol/mL) in a volume of 32 μL was added to 400 μL of PRP that had been incubated at 37°C for 2 min. The extent of collagen-induced platelet aggregation was quantitated by measuring the percent maximal aggregation 6 min after adding collagen. Data are expressed as means ± SD, n = 3 (α-tocopherol), 3 (α-tocotrienol), and 4 (TRF) dogs respectively, per treatment. Figure 2A is based on raw values and 2B is based on percentages compared to their respective pre-dose and post-dose control values. An asterisk indicates significant differences at P < 0.001 for each treatment compared to respective controls.