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. 2011 May 17;5(5):e1028. doi: 10.1371/journal.pntd.0001028

Figure 1. O. tsutsugamushi replication within monocytes.

Figure 1

Monocytes and L929 cells were infected with O. tsutsugamushi (two viable bacteria per cell) for different periods of time. A. The number of bacterial DNA copies was determined using qRT-PCR. The data are expressed as the mean ± SD of two independent experiments performed in triplicate. B. Monocytes were infected with O. tsutsugamushi, and the bacteria were detected using indirect immunofluorescence. C. Monocytes infected with O. tsutsugamushi for 5 days were labeled with bodipy phallacidin to detect filamentous actin and bacteria were detected using indirect immunofluorescence (in red). One representative micrograph performed in confocal microscopy is shown.