Figure 3. Quantitative RT-PCR of selected genes in stimulated monocytes.

Monocytes were stimulated with or without O. tsutsugamushi for 8 (A) and 24 (B) hours. RNA was extracted, and qRT-PCR was performed on 16 genes involved in the immune response that were differentially expressing in the microarray experiments. The results, expressed as the log₂ ratio of fold changes, are presented as the mean ± SEM of three experiments performed in triplicate.