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| Time (hours) | control | O. tsutsugamushi | heat-killed O. tsutsugamushi |
| 6 | not detectable | not detectable | not detectable |
| 24 | <1% | 4±1% | <1% |
| 48 | <1% | 8±1% | <1% |
Monocytes were incubated with O. tsutsugamushi for different periods. Apoptosis was revealed by TUNEL assay and fluorescence microscopy. The results expressed as the ratio of TUNEL-positive cells and DAPI-stained nuclei ×100 are the mean ± SD of three different experiments.
