Figure 1.

Characterization of the anti-Polaris antisera. (A) Specificity of the anti-Polaris antisera was evaluated by immunoprecipitation of in vitro translated Polaris. The immune serum immunoprecipitated a protein product of the expected size, whereas no product was seen using the corresponding preimmune serum. (B) Specificity of the antibodies was tested on Western blots by using mutant BroF2 liver cells transfected with the Tg737 expression cassette. A 95-kDa product corresponding to full-length Polaris was seen only in the transfected cells (BroF2 + Tg737) but not in nontransfected cells (BroF2).