Figure 5.

Tg737 and Polaris expression is associated with ciliated epithelium in the efferent duct. (A) Tg737 expression (blue) in the efferent duct appears to be restricted to the epithelium with no activity detected in the interstitial cells. (B) Similar to the results for the β-galactosidase assay, Polaris (red) was detected in the epithelium. These epithelial cells contain multiple cilia, as revealed by costaining with β-tubulin (green). (C and D) Higher magnification of the boxed region in B shows that Polaris is concentrated at the apical surface of the cells and extends into the cilia axonemes.