Figure 7.

Tg737 and Polaris expression and localization in ciliated cells in the brain. (A) High levels of Polaris (red) expression were detected near the apical surface of ependymal cells lining the ventricles of the brain with significantly lower expression in cells of the choroid plexus. (B and C) Ependymal cells are extensively ciliated as revealed by β-tubulin (green) expression. Polaris was found at the base of each cilium. Identical localization was seen in the choroid plexus (B, inset) where short cilia were detected emerging off a small domain of Polaris. (D) With the β-galactosidase reporter gene, high levels of Tg737 expression were seen in the ependymal cells with lower expression in the choroid plexus. (E and F) In addition to the epithelial cells lining the ventricles (Vn) and in the choroid plexus (Cp), Tg737 expression was seen in the Purkinje (P) cells in the cerebellum (E) and in the cells of the hippocampus (Hp) and the dentate gyrus (Dg) (F).