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. 2011 May 17;6(5):e19077. doi: 10.1371/journal.pone.0019077

Figure 8. miR-132 Knockdown Correlates to Increased Expression of Molecules Regulated by Immune Signaling Pathways.

Figure 8

miR-132 was knocked down in PC12 cells infected with the miR-132 sponge virus (Figure 4). Whole rat genome microarrays were probed to screen for changes in miR-132 knockdown cells vs. control-infected cells. a, Functional grouping of genes that increased when miR-132 was knocked down indicated a significant enrichment in genes regulated by signaling pathways that are involved in immune function. The pie chart represents all categories for the upregulated genes. The labeled categories are the 8 most significantly enriched. b, Using real-time PCR we confirmed an increase in 4 out of 5 genes that were upregulated on the microarray. c, At 21 days post-injection miR-132 sponge expressing neurons (green) had an increase in IL-6 immunofluorescence (compare blue in white outlined areas in 3rd panel from the left) as compared to control redRubi infected neurons (red), consistent with the result for IL-6 in the microarray.