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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Cancer Res. 2011 Apr 6;9(5):589–602. doi: 10.1158/1541-7786.MCR-10-0565

FIGURE 3.

FIGURE 3

DNA-damage response also contributes to the constitutive levels of the IFI16 protein. A, Sub-confluent cultures of young WI-38 or IMR-90 HDFs were either left untreated (lanes 1 and 4) or treated with increasing units of bleomycin (lanes 2, 3, 5 and 6) for 24 h. After the treatment, total cell extracts were analyzed by immunoblotting for the indicated proteins. B, Sub-confluent cultures of young WI-38 were either left untreated (lanes 1) or treated with increasing units of bleomycin (lanes 2 and 3) for 24 h. After the treatment, total cell extracts were analyzed by immunoblotting for the indicated proteins. C, Sub-confluent cultures of young WI-38 were either left untreated or treated with bleomycin for 24 h. After the treatment, total RNA was analyzed by quantitative real-time PCR for IFI16 and AIM2 mRNA levels. The ratio between the test gene (the IFI16 or AIM2) mRNA levels to actin mRNA was calculated in units (one unit being the ratio of the test gene to actin mRNA). The relative steady-state levels of IFI16 mRNA in control HDFs are indicated as 1.