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. Author manuscript; available in PMC: 2012 May 15.
Published in final edited form as: Clin Cancer Res. 2011 Feb 15;17(10):3478–3489. doi: 10.1158/1078-0432.CCR-10-2372

Figure 2. Invasiveness and tumor cell autophagy in aggressive and indolent melanoma cell lines.

Figure 2

(A) Invasion rate by the Boyden Chamber assay (B) Autophagy modulation with the autophagy inhibitor chloroquine (CQ) or the autophagy inducer rapamycin (Rap) in cell lines expressing the autophagy marker GFP-LC3. Diffuse fluorescence: no autophagy; punctate fluorescence: accumulation of autophagic vesicles. (C) Immunoblotting against autophagy markers in aggressive (SKMEL28, C8161) and indolent (WM3918, A375P) cell lines grown in complete medium:Replete; and in serum free (24 hours) and nutrient free medium (2 hours):SF/N deprived. (D) Gel densitometry of autophagy markers in replete and SF/N deprived media. Fold change of markers in SF/N deprived conditions were compared to Replete measurements.