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. Author manuscript; available in PMC: 2012 May 15.
Published in final edited form as: Clin Cancer Res. 2011 Feb 15;17(10):3478–3489. doi: 10.1158/1078-0432.CCR-10-2372

Figure 4. Growth, invasion, and tumor cell autophagy of aggressive and indolent melanoma spheroids grown in a three dimensional collagen matrix.

Figure 4

(A) Brightfield microscopy of spheroids; black arrow: central spheroid boundary; red arrow: boundary of invasion (B) immunoblots of lysates derived from spheroids 48 hours after implantation in collagen; mean +/− SD gel densities for indolent (WM3918, A375) and aggressive (SKMEL28, C8161). (C) Brightfield and fluorescent microscopy of A375P GFP-LC3 and C8161GFP-LC3 spheroids (48 hours) (D) Electron micrographs of A375P and C8161 spheroids (48hours); arrows: autophagic vesicles. (D) Live (green)/Dead (orange) assay of melanoma spheroids in 3D culture 48 hours after the indicated treatments, HCQ: hydroxychloroquine; Scale bar: 200 μm (A,C,E); 2 μm (D).