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. 2011 Mar 11;18(6):390–398. doi: 10.1038/cgt.2011.4

Figure 1.

Figure 1

Effect of prodrugs on tumors that express E. coli PNP (purine nucleoside phosphorylase) in 10% of the cells. Wild-type D54 tumor cells were mixed with D54 tumor cells that had been transduced with the E. coli PNP gene, so that 10% of the mixture contained cells that expressed E. coli PNP. This 90/10 mixture was injected subcutaneously into the flanks of nude mice. Drug treatment began on day 14 when tumors were approximately 300 mg. The activity of E. coli PNP and human adenine phosphoribosyl transferase in the tumors at the time of treatment were 11 200±2300 units and 87±30 units, respectively. In F-araAMP-treated mice on day 66 there was one complete regression, two small non-growing tumors and three small (<500 mg) but growing tumors. The growth of tumors in mice treated with drugs was significantly different than that in vehicle treated animals; P values⩽0.001 in all cases. The experiment has been repeated with similar results.