Fig. 2.

PXR activity in presence of extra-hepatic EHS tumor. Male mice harboring the −13 kb CYP3A4/lacZ transgene were treated with corn oil vehicle or PCN as described in the experimental procedures. A. Hepatocytes exhibiting transgene expression are visualized as the blue stained areas on the cut liver surface after X-gal histochemical staining. B. Transgene expression as determined by β-galactosidase activity in total liver lysates using the ONPG assay. The units of β-galactosidase activity are given as absorbance at 420nm per milligram of protein per minute. C. Basal and PCN-induced mouse endogenous Cyp3a11 mRNA expression in livers of control and tumor-bearing animals. Graphs express the mean ± SEM for n = 8/9 animals per group. (*** p ⪡ 0.001, * p < 0.05)