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. Author manuscript; available in PMC: 2012 May 15.
Published in final edited form as: Clin Cancer Res. 2011 Apr 15;17(10):3170–3180. doi: 10.1158/1078-0432.CCR-10-3289

Fig. 3.

Fig. 3

CAR activity in presence of extra-hepatic EHS tumor. Male mice harboring the −13 kb CYP3A4/lacZ transgene were treated with corn oil vehicle or TCPOBOP as described in the experimental procedures. A. Hepatocytes exhibiting transgene expression are visualized as the blue stained areas on the cut liver surface after incubation with X-gal. Staining intensity reflects the degree of CYP3A4 transgene expression. B. CYP3A4/lacZ transgene expression as determined by β-galactosidase activity in total liver lysates using the ONPG assay. The units of β-galactosidase activity are given as the absorbance at 420nm per milligram of protein per minute. C. Basal and TCPOBOP-induced mouse endogenous Cyp3a11 mRNA expression in livers of control and tumor animals. D. Basal and TCPOBOP-induced mouse endogenous Cyp2b10 mRNA expression in livers of control and tumor animals. Graphs express the mean ± SEM for n = 8/9 animals per group. (*** p ⪡ 0.001, * p < 0.05)