Skip to main content
. Author manuscript; available in PMC: 2012 May 15.
Published in final edited form as: Clin Cancer Res. 2011 Apr 7;17(10):3219–3232. doi: 10.1158/1078-0432.CCR-11-0234

Figure 4. HDACIs and KW2449 induce DNA damage and ROS in Bcr/Abl+ cells, events attenuated by the ROS scavenger Mn-TBAP.

Figure 4

Figure 4

A) Following treatment as described in Figure 3, protein lysates were obtained and WB performed using γH2A.X antibodies. B) K562 cells were pretreated with 20 µM ZVAD-fmk for 2 hr followed by KW/vorinostat as described above for 30 hr. At the end of this period, the cells were lysed and subjected to WB (NS: non-specific). C) K562 cells were incubated with KW, vorinostat, SNDX alone or in combination for 24 hr, after which cells were labeled with CM-H2DCF-DA or HE for flow cytometric analysis. Values represent the means ± S.D. for triplicate determinations, (* P<0.05). D) K562 were exposed to either KW+vorinostat or KW+SNDX in the presence or absence of the free radical scavenger Mn-TBAP (300 µmol/L) for 72 h. The cells were collected and analyzed by flow cytometry to determine the percentage of 7AAD positive cells. E) Cells treated as in (D) were collected at 30 hr and Western blot analysis performed. Two additional experiments yielded equivalent results.