Table 5.

Comparison of kinetic parameters for various sGC β1 mutants in α1β1 and H-NOX domains at 37 °C

Protein	Mutation	obs Kon O2 (μM−1 s−1)	Kox (s−1)
α1β1	wt	N.O.a	N.O.
	β1 P118A	N.O.	N.O.
	β1 I145Y	N.O.	N.O.
β1(1–385)	wt	N.O.	0.00029 ± 0.00002
	β1 P118A	N.O.	17.6 ± 0.3b
	β1 I145Y	~0.00004c	0.00020c
	β1 P118AI145Y	0.00012 ± 0.00003	0.00062 ± 0.00025
β1(1–194)	wt	N.O.	0.00121d
	β1 P118A	N.O.	0.1950 ± 0.006
	β1 I145Y	N.O.	0.0022 ± 0.0005

^aN.O., not observed;

^bRate measured and reported at 10 °C;

^cRates from (32);

^dRates from (6).