Figure 5. Trisomic tumors retain higher dosage for chromosome 16.
A, Trisomic and euploid gene copy number was determined using Taqman assay. Normalized ratios of Mmu16 genes (Jam2, Rcan1 and Mx1) to control genes (Apob) were elevated in samples from both trisomic normal brain and tumor samples, indicating that extra Mmu16 information is retained in the tumors (*P < 0.05). B, Cells recovered from a disaggregated sarcoma that arose in Ts65Dn retain three copies of distal Mmu16 by FISH (more than 50 cells counted).