Table 1.
Mössbauer parameters and percent absorptions of Fe cofactors in the as-purified, ascorbate-reduced, and dithionite-reduced cNOR from Ps. nautica
| Cofactor | δ (mm/s) | ΔEQ (mm/s) | As-purified | Absorption in percent | |
|---|---|---|---|---|---|
| Ascorbate-reduced | Dithionite-reduced | ||||
| FeBII | 1.10 | 2.68 | 8 (1) | 11 (1) | 15 (2) |
| FeBIII | 0.25 | 0.59 | 17 (2) | 14 (2) | 10 (1) |
| FeII-heme b3 | 0.49 | 1.17 | 0 | 0 | 15 (2) |
| FeIII-heme b3 | 0.25 | 1.99 | 25 (3) | 25 (3) | 10 (1) |
| FeII-heme b | 0.43 | 1.13 | 3 (1) | 25 (3) | 25 (3) |
| FeIII-heme b | 0.23 | 2.04 | 22 (2) | 0 | 0 |
| FeII-heme c | 0.43 | 1.15 | 3 (1) | 25 (3) | 25 (3) |
| FeIII-heme c | 0.17 | 1.61 | 22 (2) | 0 | 0 |
The values in parentheses give the uncertainties in the last significant digits estimated for the percent absorptions. Due to the complexity of the spectra, the Mössbauer parameters (δ and ΔEQ) were fixed during the least-squares fittings. The line-widths of the quadrupole doublets used in our fits are 0.28 mm/s for FeII-heme b and FeII-heme c, 0.4 mm/s (left line) and 0.3 mm/s (right line) for FeIII-heme b, 0.3 mm/s (left) and 0.5 mm/s (right) for FeIII-heme c, 0.28 mm/s (left) and 0.35 mm/s (right) for FeII-heme b3, 0.36 mm/s (left) and 0.43 mm/s (right) for FeIII-heme b3, 0.35 mm/s (left) and 0.3 mm/s (right) for FeBII.