TABLE 1.
Release of specific proteins from permeabilized cellsa
| Protein | Leakage (%)
|
|
|---|---|---|
| Permeabilized cells | Permeabilzed cells plus latrunculin | |
| DNA polymerase | <1 | 5 |
| Citrate synthase | 4 | 10 |
| Glucose 6-phosphatase | <1 | 5 |
| Leu-tRNA synthetase | 1 | 75 |
| Gly-tRNA synthetase | 10 | 81 |
| Lys-tRNA synthetase | 1 | 85 |
| Glucose 6-P dehydrogenase | 8 | 43 |
| Lactate dehydrogenase | 40 | 78 |
| Glyceraldehyde 3-P dehydrogenase | 34 | 80 |
a
To determine release, enzyme assays were carried out on supernatant fractions and on intact cells as described in Materials and Methods. Percent leakage is defined as the amount of activity in the supernatant fraction (corrected for cell loss) divided by the activity in an equivalent amount of intact cells. The data represent the averages of at least two experiments.