Fig. 4.

Subcellular localization of Rinl and co-localization with Rab5a and actin. (A) myc-tagged Rinl was transiently expressed in COS7 cells and stained with antibodies against Rinl and myc. Representative confocal images are shown. Scale bar, 25 μm. Transfected cells were assayed for their Rinl expression patterns. Expression patterns were divided into four categories. A quantification is shown (n > 100). Error bars, s.e.m. (B) GFP-Rab5a S34N alone or together with myc-tagged Rinl were co-expressed in COS7 cells. Scale bar, 25 μm. A quantification of the Rab5a S34N distribution was performed as described in A (n = 50). Error bars, s.e.m. (C) Transiently transfected COS7 cells were treated with cytochalasin D or DMSO for 30 minutes. Cells were stained with anti-myc antibodies to detect Rinl and with TRITC-conjugated phalloidin to label the actin cytoskeleton. Representative confocal images are shown. Scale bar, 25 μm.