Fig. 6.

Rab22 and Rinl co-localize to actin-positive membrane ruffles. (A) GFP-Rab22 S19N alone or together with myc-tagged Rinl were expressed in COS7 cells. Representative confocal images are shown. Scale bar, 25 μm. Rab22 expression, when transfected alone or together with Rinl, was assayed by confocal microscopy. Subcellular expression patterns were quantified as described in Fig. 5. n > 50; Error bars, s.e.m. (B) Transiently transfected COS7 cells were treated with cytochalasin D or DMSO for 30 minutes. Cells were stained with anti-myc antibodies to detect Rinl and with TRITC-conjugated phalloidin to label the actin cytoskeleton. Scale bar, 25 μm.