Fig. 8.

Rinl increases the internalization of HRP and EGFR. (A) HEK 293T cells expressing GFP-Rinl, GFP-Rab5a wt, GFP-Rinl and GFP-Rab5a wt or GFP alone were loaded with HRP. HRP uptake was quantified from two independent experiments done in triplicates. Error bars, s.e.m. (B) HeLa cells were transfected with GFP, GFP-Rinl, GFP-Rab5a wt, GFP-Rinl and GFP-Rab5a wt or GFP-Rab5a S34N. Cells were incubated with Alexa 555-conjugated EGF at 4 °C and EGF uptake at 37 °C was imaged after 5 and 15 minutes. Representative confocal images are shown. Scale bar, 25 μm. The GFP signals of the equivalent cells are shown in Fig. S8.