Figure 1.

Coimmunoprecipitation of GIPC with TrkA and TrkB. Full-length GIPC and TrkA or TrkB were transiently coexpressed in HEK293T cells, followed by immunoprecipitation (IP) of cell lysates (50 μg) with affinity-purified anti-Trk (C-14) IgG and immunoblotting (IB) with anti-GIPC (top) and anti-Trk (44) serum (middle). GIPC coprecipitates with both TrkA (lane 2) and TrkB (lane 3). No GIPC is detected in immunoprecipitates from lysates of cells transfected with GIPC and an empty vector (lane 1). The protein expression level of GIPC is comparable in each lane (bottom).