Table 2. A–85380 But Not PNU–282987 Increases GAD₆₇ Protein Expression in Mouse Frontal Cortex.

A–85380 (mg/kg)	GAD67a	GAD65a
VEH	0.23±0.05	0.45±0.07
0.5	0.52*±0.05	0.47±0.10
1	0.37*±0.03	0.41±0.05
2.5	0.35*±0.02	0.53±0.08
PNU–282987 (mg/kg)	GAD67a	GAD65a
VEH	0.21±0.03	0.47±0.06
1	0.26±0.02	0.41±0.03
2.5	0.17±0.01	0.54±0.05
5	0.25±0.02	0.38±0.04

Mice were treated s.c. with A–85380 or PNU–282987 two times a day for 5 days. GAD₆₇ and GAD₆₅ were measured 2 h after the last injection of drug. Overall one-way ANOVA for GAD₆₇ in VEH-, A–85380-, and PNU–282987-treated mice (F_{6, 6}=5.4) yielded a p<0.01 in the FC.

^*p<0.01 for Student–Newman–Keuls multiple comparison between VEH- and A–85380- treated mice.

^aData is expressed as GAD₆₇ or GAD₆₅/β-actin OD ratio.