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. 2003 Dec;23(24):9117–9126. doi: 10.1128/MCB.23.24.9117-9126.2003

FIG. 3.

FIG. 3.

Analysis by IEF-SDS-PAGE of d4E-BP phosphorylation. (A) Selected S2 cell extracts for IEF-SDS-PAGE (arrows). Rapamycin-treated cell extract (not shown) is similar to unstimulated cell extract. (B to F) IEF-SDS-PAGE of extracts from serum-starved (B), insulin-treated (15 min) (C), insulin-treated (30 min) (D and E), and rapamycin- and insulin-treated (15 min) (F) cells were probed with the indicated antibodies. d4E-BP (B to F) was assessed by blotting it with anti-d4E-BP (αd4E-BP) or anti-phospho-4E-BP1(Thr37/46) (α4E-BP1 P-T37/46) as indicated. The individual isoforms are represented by the letters a to e. P, phosphorylated.