Figure 4.

Toluene inhibition of AMPA EPSCs requires postsynaptic Ca²⁺ and the CB1 receptor. (a) Traces are representative examples of AMPA-mediated EPSCs recorded under baseline conditions (left) and following exposure to toluene (right). Normal aCSF and internal solution, BAPTA in the internal solution, AM-281 in the aCSF, and MPEP in the aCSF. (b) Time-course of AMPA-mediated EPSCs during toluene exposure. Data are expressed as a percent of the averaged pre-toluene response under each condition. ^***Values significantly different when recording with intracellular BAPTA or extracellular AM-281, compared with normal aCSF and internal solution. (p<0.001, two-way ANOVA) (c) Summary of effects of toluene on AMPA-mediated currents under various experimental conditions. ^**Values significantly different from baseline amplitudes. (p<0.01, repeated measures ANOVA, post hoc Dunnett's multiple comparison test). Data are mean±SEM. The color reproduction of this figure available on the HTML full text version of the paper.