FIG. 3.
In type II survival, Clb2 is not required for recombination of TG1-3 sequences but rather is required for operation of the Rad50/Rad59 pathway of recombination. Cells of the relevant indicated genotype were restreaked on a YEPD plate immediately after sporulation, grown for 3 days at 25°C, and photographed. In all three viable strains, fast-growing colonies appeared on the first day following restreaking (not shown). The telomere structure (XhoI digestion, TG1-3 32P-labeled probe) is shown for two fast-growing colonies, representative of a total of 12, for two strains only, picked out individually at day 1. All of were type II recombinants (amplification of the TG1-3 sequences), as explained in the legend to Fig. 1B. On the plates shownhere, streaking approximately equal amounts of cells for all four strains allowed for the visualization of the much higher viability of the cdc13-1 tlc1Δ strain compared to the two viable clb2Δ strains.