Table 1.

Primer and probe sequences used in qRT-PCR for PAPPA2, PAPPA, ADAM12, E-cadherin, β-actin and GAPDH

Gene	Forward Primer	Reverse Primer	Annealing Temperature	Source
PAPPA2	ACTCACCCAAGAGGGCATACATGA	GCACTGAGCTGGCAAAGTAGATGT	50°C	This paper
PAPPA	GTCATCTTTGCCTGGAAGGGAGAA	AGGGCTGTTCAACATCAGGATGAC	56°C	This paper
ADAM12	CTGGGCCACAATTTCGGGATGAAT	ACTGCTGAACACCATGGGAAATGG	50°C	This paper
E-cadherin	AGCCTCTGGATAGAGAACGCATTG	GGGTGAATTCGGGCTTGTTGTCAT	50°C	This paper
β-actin	GCGAGAAGATGACCCAGGATC	CCAGTGGTACGGCCAGAGG	60°C	Knerr et al. (2005) [31]
GAPDH	CGGGAAGCTTGTGATCAATGG	GGCAGTGATGGCATGGACTG	56°C	Kudo et al. (2003) [30]