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. Author manuscript; available in PMC: 2012 May 25.
Published in final edited form as: Virology. 2011 Apr 22;414(1):10–18. doi: 10.1016/j.virol.2011.03.026

Fig. 4. BMS-790052 alters the subcellular fractionation patterns of NS proteins.

Fig. 4

Huh7.5 cells were infected with a vaccinia virus expressing a T7 RNA polymerase and then transfected with a plasmid (Bart79I) containing the NS viral proteins downstream of a T7 promoter. Transfected cells were incubated with 0.1 % of DMSO (A) or 10 nM of BMS-790052 (B) for 9 hours. Membrane-enriched cell lysates were prepared and fractionated on idioxanol density gradients. Distribution patterns of NS5A, NS3, and E-cadherin proteins were examined by western blot analysis using anti-NS5A, anti-NS3, and anti-E-cadherin antibodies.