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. Author manuscript; available in PMC: 2012 May 25.
Published in final edited form as: Virology. 2011 Apr 22;414(1):10–18. doi: 10.1016/j.virol.2011.03.026

Fig. 5. BMS-790052 affects neither the in vitro replicase activity of pre-assembled RCs nor the self-dimerization of NS5A proteins.

Fig. 5

(a) Effects of BMS-790052 on an in-vitro replicase activity from pre-assembled RCs from genotype 2a. 10 μM of 3′-dCTP, 0.1 % of DMSO, 1 or 10 μM of BMS-790052 was incubated with a crude membrane fraction isolated from Huh7.5 cells harboring replicating J6/JFH full-length replicons derived from genotype 2a. Newly replicated HCV RNAs were separated on an agarose gel and exposed to a phosphorimager.

(b) Effects of BMS-790052 on the dimerization of NS5A proteins. Equal amounts of pAct-NS5A, pBind-NS5A and pG5-Luc were transfected into Huh7.5 cells. Transfected cells were incubated with 0.1 % of DMSO or 10 nM of BMS-790052. Firefly luciferase assay was performed to measure levels of interaction between two NS5A fusion proteins. pAct and pBind are negative control vectors and pAct-Myo and pBind-ID are positive control vectors.