Table 1.
Primers for this study
| Name | Sequence | Application |
|---|---|---|
| F-L-LL | 5'-GTATTGTCAggagctgtgaAGattgacacagcccAAAGCTTGCTGCACAG-3' | PCR mutagenesis for mutant L-LL |
| R-L-LL | 5'-CTGTGCAGCAAGCTTTgggctgtgtcaatCTtcacagctccTGACAATAC-3' | |
| F-L-RR | 5'-GTATTGTCAggagctgtgatcattCTcacagcccAAAGCTTGCTGCACAG-3' | PCR mutagenesis for mutant L-RR |
| R-L-RR | 5'-CTGTGCAGCAAGCTTTgggctgtgAGaatgatcacagctccTGACAATAC-3' | |
| F-L-RL | 5'-GTATTGTCAggagctgtgaAGattCTcacagcccAAAGCTTGCTGCACAG-3' | PCR mutagenesis for mutant L-RL |
| R-L-RL | 5'-CTGTGCAGCAAGCTTTgggctgtgAGaatCTtcacagctccTGACAATAC-3' | |
| F-S-LL | 5'-GCCTTGACATTTGTATTGTCAggagctgtgatcattctgtgtcgggAAAGCTTGCTGCACAGAAAC-3' | PCR mutagenesis for mutant S-LL |
| R-S-LL | 5'-GTTTCTGTGCAGCAAGCTTTcccgacacagaatgatcacagctccTGACAATACAAATGTCAAGGC-3' | |
| F-S-RR | 5'-GCCTTGACATTTGTATTGTCAccacgacacaagattgacacagcccAAAGCTTGCTGCACAGAAAC-3' | PCR mutagenesis for mutant S-RR |
| R-S-RR | 5'-GTTTCTGTGCAGCAAGCTTTgggctgtgtcaatcttgtgtcgtggTGACAATACAAATGTCAAGGC-3' | |
| F-S-RL | 5'-GCCTTGACATTTGTATTGTCAcccgacacagattctagtgtcgaggAAAGCTTGCTGCACAGAAAC-3' | PCR mutagenesis for mutant S-RL |
| F-S-RL | 5'-GTTTCTGTGCAGCAAGCTTTcctcgacactagaatctgtgtcgggTGACAATACAAATGTCAAGGC-3' | |
| F-EX | 5'-GCCTTGACATTTGTATTGTCAtggaggcgtgggtacagccccgccccaAAAGCTTGCTGCACAGAAAC-3' | PCR mutagenesis for mutant EX |
| R-EX | 5'-GTTTCTGTGCAGCAAGCTTTtggggcggggctgtacccacgcctccaTGACAATACAAATGTCAAGGC-3' | |
| F-D1 | 5'-GACATTTGTATTGTCAgagctgtgatcattgacacagccAAAGCTTGCTGCACAG-3' | PCR mutagenesis for mutant D1 |
| R-D1 | 5'-CTGTGCAGCAAGCTTTggctgtgtcaatgatcacagctcTGACAATACAAATGTC-3' | |
| F-D2 | 5'-GACATTTGTATTGTCAgctgtgatcattgacacagcAAAGCTTGCTGCACAG-3' | PCR mutagenesis for mutant D2 |
| R-D2 | 5'-CTGTGCAGCAAGCTTTgctgtgtcaatgatcacagcTGACAATACAAATGTC-3' | |
| F-D3 | 5'-GCCTTGACATTTGTATTGTCActgtgatcattgacacagAAAGCTTGCTGCACAGAAAC-3' | PCR mutagenesis for mutant D3 |
| R-D3 | 5'-GTTTCTGTGCAGCAAGCTTTctgtgtcaatgatcacagTGACAATACAAATGTCAAGGC-3' | |
| F-D4 | 5'-GCCTTGACATTTGTATTGTCAtgtgatcattgacacaAAAGCTTGCTGCACAGAAAC-3' | PCR mutagenesis for mutant D4 |
| R-D4 | 5'-GTTTCTGTGCAGCAAGCTTTtgtgtcaatgatcacaTGACAATACAAATGTCAAGGC-3' | |
| F-D5 | 5'-GCCTTGACATTTGTATTGTCAgtgatcattgacacAAAGCTTGCTGCACAGAAAC-3' | PCR mutagenesis for mutant D5 |
| R-D5 | 5'-GTTTCTGTGCAGCAAGCTTTgtgtcaatgatcacTGACAATACAAATGTCAAGGC-3' | |
| F-D6 | 5'-GCCTTGACATTTGTATTGTCAtgatcattgacaAAAGCTTGCTGCACAGAAAC-3' | PCR mutagenesis for mutant D6 |
| R-D6 | 5'-GTTTCTGTGCAGCAAGCTTTtgtcaatgatcaTGACAATACAAATGTCAAGGC-3' | |
| Qst | 5'-GAGTGACGAGGACTCGAGCGCATGCTTTTTTTTTTTTTT-3' | RT for cDNA preparation |
| F-6 | 5'-GTATAGGTGTTGGCTCTATGC-3' | (-) gRNAs analysis |
| R-683 | 5'-GGAGCGGCAGGTTGGTTAACACG-3' | (-) gRNAs analysis |
| F-12 | 5'-GTGTTGGCTCTATGCCTTGAC-3' | (-) gRNAs analysis, sg mRNA7 analysis |
| R-343 | 5'-ATAGAATAGGCCCAGCACCCC-3' | (-) gRNAs analysis |
| R-15284 | 5'-CTCCACAGTGTAACTTATCCTCC-3' | sg mRNA7 analysis |
| F-actin | 5'-CCCATCTATGAGGGCTACGC-3' | β-actin analysis |
| R-actin | 5'-TTTGATGTCACGCACAATTTC-3' | β-actin analysis |
Prefixes: F, forward primer; R, reverse primer; RT, reverse transcription; (-) gRNAs, negativ-strand genomic RNA; sg, subgenomic. The lowercase represent different sequences compared the APRRSV (GQ330474) in N-SL2.