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Endogenous Tiam1 localizes to the Golgi apparatus in breast cancer cells. (a) Cell lines were plated on collagen I (50 μg/ml)-coated coverslips overnight, and then fixed and stained for Tiam1 (green), GM130 (red; Golgi marker), and DAPI (blue). (b) Localization of endogenous Tiam1 in MDA-MB-231 cells also was examined in the presence or absence of blocking peptide.
