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Brefeldin A disrupts the cellular distribution of Tiam1. Cells were cultured in the presence (10 μg/ml; BFA+) or absence (BFA-) of Brefeldin A for 30 minutes under serum-starved conditions. Cells were allowed to recover for an additional 3 hours and were then fixed and stained for Tiam1 (green), GM130 (red), and DAPI (blue).
