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. 2011 Mar 7;5:17. doi: 10.3389/fnana.2011.00017

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Copyright © 2011 Berlanga, Phan, Bushong, Wu, Kwon, Phung, Lamont, Terada, Tasdizen, Martone and Ellisman.

This is an open-access article subject to an exclusive license agreement between the authors and Frontiers Media SA, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.

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Aligning serial sections using Mogrifier. Two serial coronal sections (A,B) are shown as they would appear in Mogrifier (i.e., in red and green channels). Image (B) illustrates an example of non-uniform compression of the lateral ventricle in a tissue section. Image (C) shows the overlay of slices (A,B) in the application Mogrifier before applying any transformation. Image (D) shows the overlay of slices (A,B) and the reference points (blue) after applying the thin plate spline transformation in Mogrifier.