Figure 6.

Aligning serial sections using ir-Tweak. Image (A) shows a wide-field view of overlaid adjacent slices in the application ir-Tweak (reference points shown as white open circles) before applying any transformation. Image (B) shows a high-magnification image of the hippocampus from (A). This image demonstrates the mis-alignment of serial sections before applying the thin plate spline transformation in ir-Tweak. Image (C) shows the same high-magnification image from (B) after applying the thin plate spline transformation in ir-Tweak.