Figure 1.

FACS analysis of T cell marker from spleen and liver CD3⁺/DX5⁺NKT cells in Balb/c mice. DX5⁺NKT cells were isolated from spleen and liver of Balb/c mice using MACS and FACS-Sorting. Representative dot plot (A). Expression of different T cell markers revealed distinct subsets in Balb/c mice. In the spleen, more DX5⁺NKT cells appeared CD4⁺, in the liver, they expressed more Vβ8.1/8.2 (B). Upon 4 h activation with anti-CD3 and anti-CD28, spleen DX5⁺NKT cells displayed a TCR_α/β down-regulation, whereas liver DX5+NKT cells did not (C). Results are given as mean + SEM. Experiments were repeated at least three times (* p < 0.05).