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. 2011 Apr 29;12:26. doi: 10.1186/1471-2172-12-26

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Copyright ©2011 Werner et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cytokine production by spleen DX5⁺NKT cells is different in NK1.1^-Balb/c compared to the NK1.1⁺mice strain C57Bl/6. Isolated DX5⁺NKT cells were stimulated with antibodies against CD3 and CD28 for 4, 24 and 48 h. FACS-Analysis revealed a Th1 sided cytokine profile for spleen DX5⁺NKT cells of NK1.1⁺C57Bl/6 mice, whereas spleen DX5⁺NKT cells of NK1.1^-Balb/c mice displayed a pattern with Th1 and Th2 cytokines. Results are given as mean + SEM. Experiments were repeated at least three times (* p < 0.05).